All parts of question 10 please! (BIOCHEM) 2 10 pO2 (kPa) 10. The following show
ID: 205491 • Letter: A
Question
All parts of question 10 please! (BIOCHEM)
2 10 pO2 (kPa) 10. The following shows an Fab fragment of an antibody, with the variable region on the right. Please explain how the 3-dimensional structure of this protein facilitates the ability of antibodies to develop specificity against a large number of different antigens (2 pts). Fab fragments of antibodies raised against a specific protein of interest are sometimes used in X-ray crystallography to help that protein crystallize. Suggest why this might work (1 pt). Note that for a crystal to form, you must have regular protein-protein contacts throughout the crystal. Also, what could be another workaround to solve the 3-dimensional structure of a protein that cannot be crystallized (1 pt)?Explanation / Answer
10.. the Fab region on antibodies are region where the antigen binds. Fab region made up of one variable region of heavy chain and light chain. 3D structure of this antibody facilitate the ability of antibody to bind with different antigen is via epitope structure of antigen epitope is specific structure present on antigen that will bind to the Fab region of antibody. By this mean antibody may react with different antigen through the structure of epitopes.
Crystralization is used to study 3D structure of protein. Without it protein can not be analyzed under X rays. For crystallization, the protein must be super saturated (highest concentration) without any aggregation and precipitation. It works because the crystal form of 3D structure of protein provides visual platform to study under X ray crystallographic technique.
Another workaround to solve 3D structure of protein is NMR spectroscoply (nuclear magnetic resonance)