In fruit flies, “GAL4-UAS” system is used to study gene expression and function.
ID: 206958 • Letter: I
Question
In fruit flies, “GAL4-UAS” system is used to study gene expression and function.
Below is a diagram that shows that when Gal4 proteins bind at the DNA sequence called “UAS” present in the upstream of the gene X, then transcription from gene X takes place at higher level. In the absence of Gal4 proteins, gene X transcript is undetectable.
The gene X has its own promoter “P” downstream of the “UAS” sequence where general transcription factors “TFs” can bind.
1b. In fruit flies, "GAL4-UAS" system is used to study gene expression and function Below is a diagram that shows that when Gal4 proteins bind at the DNA sequence called "UAS" present in the upstream of the gene X, then transcription from gene X takes place at higher level. In the absence of Gal4 proteins, gene X transcript is undetectable. The gene X has its own promoter "P" downstream of the "UAS" sequence where general transcription factors "TFs" can bind. P is the promoter GAL4 300 TFs Gal4 gene gene Increased transcription from gene X From the information, identify what kind of response sequence "UAS" is and what kind of regulatory molecule Gal4 proteins are. Explain your answer. (1.5 point)Explanation / Answer
Gene expression activation is done by UAS-Gal4 system. UAS is the acronym for upstream activation sequence. UAS-Gal4 system functions as an enhancer that is specific for the Gal4 protein. This is a biochemical method that is used for studying the gene expression and its function in the organisms. It is used to study the receptor chemical binding functions in the In Vitro conditions.
UAS-Gal4 system expresses the gene at higher levels than the endogenous promoters. Hundreds of genes in Drosophila genome are driven by the Gal4 lines. Gal80 protein is yet another protein that binds to the Gal4 and inhibits the activity of Gal4. Gal80 is temperature sensitive (active at 19 degree C and inactive at 30 degree C).