Met Phe Tyr Cys Ser Gly Asp Val His Asp 1) Prepare a mRNA transcript that the pr

Question

Met Phe Tyr Cys Ser Gly Asp Val His Asp

1) Prepare a mRNA transcript that the prokaryotic ribosome will bind and translate in to the polypeptide sequence (include and label any initiation and termination sequence required for ribosomal function) (5 pts)

2) Prepare a mRNA transcript that a eukaryotic ribosome will bind and translate into the above polypeptide sequence (include and label any sequences involve in initiation, termination and stablization of this transcript) (5 pts)

3) Choose either mRNA transcript and reverse transcribe into a double strand DNA sequence (label the coding and template strands) (5 pts)

I already asked to chegg before and I got

Prokaryotic ribosome binding site = AGGAGG = Shine-Dalgarno sequence

Eukaryotic ribosome binding site = A/GCCACCAUGG = Kozak sequence

Given protein sequence: NH2-Met Phe Tyr Cys Ser Gly Asp Val His Asp-COOH

1. Prokaryotic mRNA sequence: 5'-NNAGGAGGAUGUUUUAUUGUGGGGAUGUUCAUGAUUAA-3'

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2. Eukaryotic mRNA sequence: 5'-NNGCCACCAUGGAUGUUUUAUUGUGGGGAUGUUCAUGAUUAAAAAAAA-3'

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3. DNA sequence of Eukaryotic gene

Coding strand: 5'-NNGCCACCATGGATGTTTTATTGTGGGGATGTTCATGATT-3'

Non-coding : 3'-NNCCGTGGTACCTACAAAATAACACCCCTACAAGTACTAA-5'

However, my friend brought

1. Prokaryote 5'- Rbs AUG UUU UAU UGU UCU GGU GAU GUU CAU GAU UAA - 3'

Initiation: AUG Termination: UAA

2. Eukaryote 5'- Rbs CAP AUG UUU UAU UGU UCU GGU GAU GUU CAU GAU UAA AAAA- 3'

Initiation: AUG Termination: UAA

3.     Coding strand 5’ - ATG TTT TAT TGT TCT GGT GAT GTT CAT GAT TAA -3’

Template Strand  3’ - TAC AAA ATA ACA AGA CCA CTA CAA GTA CTA ATT – 5’

as an answer. Which one is correct?

Explanation / Answer

The one you have obtained from the chegg has all information. And its the correct one.

The question itself state that to prepare a mRNA transcript that the prokaryotic ribosome will bind and translate it into the polypeptide sequence, prepare a mRNA transcript that a eukaryotic ribosome will bind and translate into the polypeptide sequence .

Prokaryotic ribosome binding site is Shine-Dalgarno sequence.

And eukaryotic ribosome binding site is Kozak sequence.

THIS BINDING SITE IS ABSENT IN THE FOLLOWING SEQUENCE,

1. Prokaryote 5'- Rbs AUG UUU UAU UGU UCU GGU GAU GUU CAU GAU UAA - 3'

Initiation: AUG Termination: UAA

2. Eukaryote 5'- Rbs CAP AUG UUU UAU UGU UCU GGU GAU GUU CAU GAU UAA AAAA- 3'

Initiation: AUG Termination: UAA

Whereas the BINDING SITE IS PRESENT IN THE FOLLOWING SEQUENCE,

Prokaryotic mRNA sequence: 5'-NNAGGAGGAUGUUUUAUUGUGGGGAUGUUCAUGAUUAA-3'

Eukaryotic mRNA sequence: 5'-NNGCCACCAUGGAUGUUUUAUUGUGGGGAUGUUCAUGAUUAAAAAAAA-3'

Binding site is found before the start codon which i have underlined.

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