Match the vocabulary term with the correct definition. Term ____ 1. Taq polymera
ID: 217937 • Letter: M
Question
Match the vocabulary term with the correct definition.
Term
____ 1. Taq polymerase
____ 2. biotechnology
____ 3. Genbank
____ 4. Restriction enzyme
____ 5. Gene cloning
____ 6. Gel electrophoresis
____ 7. Polymerase chain reaction (PCR)
____ 8. plasmid
____ 9. Recombinant DNA
____ 10. The Human Genome Project
Definition
a. An enzyme, often isolated from bacteria, that catalyzes the cutting of both strands of a double-stranded DNA molecule at a specific sequence.
b. An analytical technique in which DNA molecules are separated by size during their migration through a charged electrical field.
c. The project in which the DNA of all of the human chromosomes was sequenced.
d. The field in which biological knowledge is used in concert with technology to solve practical problems.
e. A small, circular, double-stranded DNA molecule often found in bacteria.
f. A thermostable enzyme that catalyzes primer extension in a PCR reaction.
g. The National Center for Biotechnology Information, part of the United States Government, which maintain genomic sequences (and other sequences) in a database.
h. Two or more DNA strands that combine DNA sequences which would not normally occur together; the DNA ligated together can even be from different organisms.
i. The process of isolating a DNA sequence of interest for the purpose of making multiple copies of it.
j. A cyclic process in which three main chemical reactions are repeated to create millons of copies of a target DNA sequence from starting material that might contain only a single copy of the target sequence.
*Which of the pairs of sequences below contain a single nucleotide polymorphism (SNP)?*
a. AAGGCTAA and AAGGCTAA
b. AAGGCTAA and AAGGCTGG
c. AAGGCTAA ATGGCTAA
d. GGGGGGGG and TTTTTTTT
Explanation / Answer
1. Taq polymerase- f) A thermostable enzyme that catalyzes primer extension in a PCR reaction.
2. Biotechnology- d) The field in which biological knowledge is used in concert with technology to solve practical problems.
3. GenBank- g) The National Center for Biotechnology Information, part of the United States Government, which maintain genomic sequences (and other sequences) in a database.
4. Restriction enzyme- a) An enzyme, often isolated from bacteria, that catalyzes the cutting of both strands of a double-stranded DNA molecule at a specific sequence.
5. Gene cloning- i) The process of isolating a DNA sequence of interest for the purpose of making multiple copies of it.
6. Gel electrophoresis- b) An analytical technique in which DNA molecule is separated by size during their migration through a charged electrical field.
7. Polymerase Chain Reaction(PCR)- j) A cyclic process in which three main chemical reactions are repeated to create millions of copy of a target DNA sequence from starting material that might contain only a single copy of the target sequence.
8. Plasmid- e) A small, circular, double-stranded DNA molecule often found in bacteria.
9. Recombinant DNA- h) Two or more DNA strands that combine DNA sequences which would not normally occur together; the DNA ligated together can even be from different organisms.
10. Human Genome Project- c) The project in which the DNA of all of the human chromosome was sequenced.
* The pair of sequence that contains SNP would be c) AAGGCTAA and ATGGCTAA because SNP results when there is an alteration in any single nucleotide( Adenine, Guanine, Cytosine, and Thymine). Here in this pair single adenine is converted into a thymine making it a single nucleotide polymorphism.