I think the answers are: B A F H D I C E G Practice Question Week 10 Multiple Ch

Question

I think the answers are:

B

A

F

H

D

I

C

E

G

Practice Question Week 10 Multiple Choices In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because A. the dideoxynucleotide analogs have no 2'-OH group to continue DNA synthesis B. the dideoxynucleotide analogs have no 5'-OH group to continue DNA synthesis C. the mixture is heated to 92°C to stop DNA synthesis at specific time intervals D. the dideoxynucleotide analogs have no 3'-OH group to continue DNA synthesis Matching question for DNA replication. (Match one item on the left with one function on the right). Consult the slides and the book for these answers and do a comparison with the PCR reaction as described in class. Primase DNA chain elongation B. DNA polymerase (5'-3' polymerizing activity) RNA primer synthesis C. DNA polymerase (5'-3' exonuclease activity) unwinds double-stranded DNA D. DNA polymerase (3'-5' exonuclease activity) maintains the integrity of chromosome ends E. ssDNA binding proteins Proof-reading of DNA replicatiorn F. DNA helicase joining of DNA fragments G. Topoisomerase removal of RNA primer from lagging strand H. Telomerase keep the separated DNA strands stable I. DNA ligase relieves tension in front of the replication fork

Explanation / Answer

Ans. 1 (d)

Ans 2. Match the following

A Primase -RNA primer synthesis

B DNA polymerase -proof reading  

C DNA polymerase -remove RNA primer from strands.

D DNA polymerase - chain elongation

E Ssb proteins -keep the separated strand in stable

F helicase -unwinding of DNA

G topoisomerase - relives tension in front of replication fork

I DNA ligase -joining of DNA fragments.

H telomerase -maintain the integrity of chromosomes ends.

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