Part B: Gene alignment exercise s. Using the file \"instructions for comparison

Question

Part B: Gene alignment exercise s. Using the file "instructions for comparison of HBB gene", answer the following questions Download the nucleotide sequence "human HBB.txt" and blast it against the following organisms. Write down the % identities match of the following organism's HBB (haemoglobin beta subunit- or closest homologues) with the human HBB: (8 pts) o Pan (chimpanzee taxid: 9596) (i) Dogs (taxid: 9615) (ii) Cats (taxid:9685) (ii)Mouse (taxid: 10088) (v) Chicken (taxid:9031) (vi) Salamanders (taxid:8293) vii) Snakes (taxid:8570) (vii) Fish (taxid:7898) 6. Now try to blast the HBB against the genome of Drosophila (fruit fly - taxid:7215), budding yeast (taxid 4892), grass family (taxid:4479), and paramecium (taxid: 5884) Did you find any homologues in those genomes? (4 pts) Why do you think this is the case*? (6 pts) 7. Construct a phylogenetic tree using the Clustal Omega alignment tool (Instructions in the same file). copy and paste a picture of tree into this space below (either print the tree as a pdf or take a picture and paste it back here (8 pts)

Explanation / Answer

Human HBB gene show maximum identity of 82% with dogs.

Human HBB gene show maximum identity of 84% with cats.

Human HBB gene show maximum identity of 79% with mouse

Human HBB gene show maximum identity of 77% with chicken.

Human HBB gene show maximum identity of 68% with salamander

Human HBB gene show maximum identity of 75% with snakes

Human HBB gene show maximum identity of 73% with fish

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