Please explain! Reaction in the presence of a competitive inhibitor. Reaction in
ID: 221907 • Letter: P
Question
Please explain!
Reaction in the presence of a competitive inhibitor.
Reaction in the presence of an irreversible inhibitor.
Reaction with a different substrate for which the enzyme has a lower affinity.
The following enzyme activity curves were plotted from data collected on an enzyme in the presence of different compounds. The solid line is the enzyme assayed under a standard set of conditions with no special compounds added (control). Velocity i D [S] On your Scantron sheet, bubble in the letter of the curve that is most likely to correspond to the reaction described in each question below (one letter per question, but letters may be used more than once) (3 points each) SC.1. Reaction in the presence of a competitive inhibitor. SC.2. Reaction in the presence of an irreversible inhibitor. SC 3. Reaction with a different substrate for which the enzyme has a lower affinity. SC 4. Reaction in the presence of an allosteric activator.Explanation / Answer
Answer: As per the graph
1. Reaction in the presence of a competitive inhibitor: C
2. Reaction in the presence of an irreversible inhibitor : D
3. Reaction with a different substrate for which the enzyme has a lower affinity. : D
4.Reaction in the presence of an allosteric activator : B
Enzyme inhibitor is a molecule that binds to an enzyme and decreases its activity.
Competitive inhibitors:
1) Inhibitor has affinity to the active site of enzyme where the substrate also binds
2) The substrate and the inhibitor competes
2) Vmax remains constant
Irreversible inhibitor
causes covalent modification of the enzyme,its activity is permanently reduced
Often drug's mechanism of action is like irreversible inhibitors
Allosteric activator
regulates an enzyme by binding an effector molecule at a site other than the enzyme's active site and enhances protein activity.