Part B To test the hypothesis that the three conserved regions function as regul

Question

Part B To test the hypothesis that the three conserved regions function as regulatory DNA, you make several transgenic constructs using a GFP reporter gene and you evaluate their expression in developing heart and lung tissue. Construct 1 is the wild-type construct. Notice that this constuct has normal expression in both tissues. you introduced point mutations into each of the three conserved regions (indicated by For Constructs 2-8, Expression results for each of these constructs are indicated in the chart GFP GFP in GFP in heart lung tissue tissue Construct 1 present present absent Construct 2 present absent Construct 3 present Construct 4 absent present Construct 5 absent absent absent absent Construct 6 Construct 7 absent present Construct 8 absent absent

Explanation / Answer

1. Region 1 is nessesory but not sufficient for the gene expression in heart because the expression is altered when mutation in this region. Region 3 is also required for gene expression in heart.

2.  Region 2 is nessesory and sufficient for the gene expression in lungs because the expression is altered when mutation in this region. no other region is required for gene expression in lungs.

3. Region 3 is nessesory but not sufficient for the gene expression in heart because the expression is altered when mutation in this region. Region 1 is also required for gene expression in heart.

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