Imagine that in a metabolic pathway, one of the steps involves the oxidation of
ID: 226601 • Letter: I
Question
Imagine that in a metabolic pathway, one of the steps involves the oxidation of saturated compound to an unsaturated compound, with the concomitant reduction of the cofactor FAD to FADH_2 as shown below: R-CH_2-CH_2-R' + FAD doubleheadarrow R-CH=CH-R' + FADH_2 Imagine further that Delta G degree' for this reaction is 0 kcal/mol. Given this scenario, what would be the equilibrium concentration of FADH_2 (expressed in mM to the nearest tenth of a unit) if a biochemist started with a solution that contained 0.011 M each of R-CH_2-CH_2-R', FAD, and FADH_2 (but no R-CH=CH-R'), along with 21 nM concentration of the enzyme that catalyzes the reaction?Explanation / Answer
actually it can be done by the formula.Let the reaction be
A+B--------->C+D.
delta G= delta G0' + R*T*ln {X/Y}
where R= 1.987 cal/mol k
T =temperature
X=[C]*[D].
Y=[A]*[B].