III. Effects of Temperature (4 points) (1) (2 points) Complete Table 3 below. Ef

Question

III. Effects of Temperature (4 points) (1) (2 points) Complete Table 3 below. Effects of temperature on microbial growth. 5°C 55 C 37°C Organism 7 day 20°C 2 day 7 day 2 day 7 day 2 day 7 day 2 day Negative control (sterile H o Sample #8 Sample #9 Sample #10 Sample #1 1 No growth, - slight growth, + ! ? ! moderate growth, ++; heavy growth, (2) (2 points) Based on Table 3, identify the most probable bacteria forthe bacterial samples #8-1 and justify for your conclusion. Possibilities are Escherichia coli, Saccharomyces cerevesiae, Bacillus stearothermophilus and Micrococcus luteus. Sample #8: Sample #9: Sample # 10: Sample #11 : , Justification:

Explanation / Answer

Sample #8: Micrococcus luteus

Sample #9: Saccharomyces cerevisiae

Sample #10: Bacillus stearothermophilus

Sample #11: Escherichia coli

Justifiaction: Escherichia coli is a mesophilic normally grow at 37 °C which is shown in given table. Bacillus stearothermophilus is a thermophilic and grow at the optimal temperature range (center, 65 degrees C), below it (48 and 55 degrees C), and above it (68 degrees C), in a complex medium with or without 2.5 mM Ca2+. The Ca(2+)-supplement improves growth at sub- and supraoptimal temperatures and extends it to higher temperatures

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