If the lambda bacteriophage genome is linear, how many Hind III restriction site
ID: 252760 • Letter: I
Question
If the lambda bacteriophage genome is linear, how many Hind III restriction sites does it contain? Use the sizes of fragments listed below to determine the number of fragments found after a Hind III digest.
EcoR I - Apo I 4359 Aat || - Zral 4284 BciVI 4209 BsrBI 4205 Sspl 4168 Earl 4155 Acu 1 4048 Xmn 1 3961 Hinc II 3905 Scal 3844 Bts | 3787 Bts I 3759 Pvul 3733 Pst 3607 BsrDI 3602 Asel 3537 Bsal 3433 BsrDI 3420 Cla - BspDI 23 | Hind III 29 EcoR V 185 Bmt - Nhel 229 BamHI 375 SgrA I 409 Ban II 471 Ban || 485 Btgl 528 Sph| 562 EcoN I 622 Sal |- Acc - Hinc II 651 PshAI 712 ApR TCR Eagl 939 BseYI 949 Nrul 972 BSTAPI 1045 BspM I - BfuAI 1063 Ahd 3361 Acul 3000 ori AlwNT 2884 BseYI 2777 BciVI 2682 Drd 2575 Pcil - Afl III 2473 BsrBI 2404 Ear 2351 Sap 2350 Ndel 2295/ BSTAPI 2291 BstZ171- Acc 2244 BsaAI 2225 Tth1111 - PfIF I 2217 rop PfIMI 1315 Bsm 1353 PRIMI 1364 Styl 1369 Ava - BsoB I 1425 PpuM I 1438 Mscl 1444 Btgl 1447 PpuMI 1480 Bsgl 1650 | BspEI 1664 BsaB I 1668 Xmn 2029 | Pvu || 2064 BsmBI 2122 Drd 2162Explanation / Answer
There is only one restriction site for Hind III digestion. So even if the lambda bacteriophage genome is linear you could find only one restriction site for Hind III.
After Hind III digestion you could find two fragments of DNA, a very small fragment of DNA with length of 29 and a larger one with length of 4330.