Micrococcal nuclease (MNase) is an enzyme able to cleave DNA that is not wrapped

Question

Micrococcal nuclease (MNase) is an enzyme able to cleave DNA that is not wrapped around nucleosomes. Therefore, treatment of purified chromatin with MNase allows getting fragments of DNA that were wrapped around nucleosomes and if digestion is incomplete you may obtain fragments of DNA corresponding to a single nucleosome and multiple nucleosomes.

You identified a coactivator that activates gene N and want to test if the coactivator is capable of modifying chromatin structure. You purified chromatin from wild type and a mutant in the coactivator, performed incomplete MNase treatment and Southern blot with a probe complementary to the promoter of gene N.

Based on the result above, what is most likely function of the coactivator?

Explanation / Answer

Chromatin remodelers are key players in the regulation of chromatin accessibility and nucleosome positioning on the eukaryotic DNA, thereby essential for all DNA dependent biological processes

Chromatin remodelling is most likely function of the coactivator by the Chromatin remodeling enzyme

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