Place the following steps for setting up gel electrophoresis in chronological or
ID: 254323 • Letter: P
Question
Place the following steps for setting up gel electrophoresis in chronological order.
connect the electrodes, turn on the power supply and let the gel run
add GelGreen to the agarose
submerge the gel into the buffer filled electrophoresis chamber
dissolve the agarose by heating in a microwave
pipette the samples into the wells of the gel
remove the comb from the gel
cover the gel electrophoresis chamber
after cooling for 1-2 minutes, pour the gel into the gel casting tray
connect the electrodes, turn on the power supply and let the gel run
add GelGreen to the agarose
submerge the gel into the buffer filled electrophoresis chamber
dissolve the agarose by heating in a microwave
pipette the samples into the wells of the gel
remove the comb from the gel
cover the gel electrophoresis chamber
after cooling for 1-2 minutes, pour the gel into the gel casting tray
Explanation / Answer
7
connect the electrodes, turn on the power supply and let the gel run
3
add GelGreen to the agarose
5
submerge the gel into the buffer filled electrophoresis chamber
1
dissolve the agarose by heating in a microwave
6
pipette the samples into the wells of the gel
4
remove the comb from the gel
8
cover the gel electrophoresis chamber
2
after cooling for 1-2 minutes, pour the gel into the gel casting tray
7
connect the electrodes, turn on the power supply and let the gel run
3
add GelGreen to the agarose
5
submerge the gel into the buffer filled electrophoresis chamber
1
dissolve the agarose by heating in a microwave
6
pipette the samples into the wells of the gel
4
remove the comb from the gel
8
cover the gel electrophoresis chamber
2
after cooling for 1-2 minutes, pour the gel into the gel casting tray