Please explain how to do this problem and complementation problems in general. I
ID: 255752 • Letter: P
Question
Please explain how to do this problem and complementation problems in general. I am having a hard time understanding it. TIA
note:my professor filled out the question marks so I have the answer, I just don't know how he got the answer.
Homework Problem: Difficult Question Your lab has decided to study albino fish so you gather 10 fish from various cave ponds in Mexico. You mate the fish together (don't worry about sex) and get the following results (a"" represents an albino fish and a"+" phenotype represents a fish that is wild-type in color). The "" are for you to fill in. 2 3 4 6 78910 6 10 + You are deciding which crosses to do next. Which of the following crosses will give you new information about which mutants belong to each complementation group? A. Mutant 7 x Mutant 5 B. Mutant 8 x Mutant 3 C. Mutant 7 x Mutant 3 D. Mutant 8 x Mutant 10 E. More than one of the crosses above will give you new information in the tableExplanation / Answer
Complementation tests are carried out to ascertain the location of the mutation in the genomes. When the mutations of both the mating partners are in the same gene, the mating would result in the mutant (albino) offspring.
1. Mutant 1 x Mutant 5
Observe the mating of mutant 1 with other mutant fishes and correlate the pattern with the mating of the mutant 5 with respective fishes. If the mating of both these mutants with other fishes yield the same pattern the mutation in on the same gene,
See table
Mutant 1
Mutant 5
2
-
-
3
+
+
4
+
+
6
+
+
7
+
?
8
+
+
9
+
+
10
+
+
As can be seen in the table both the fishes i.e., mutant 1 and mutant 5 have a similar pattern of offsprings when mated with other fishes. Thus, it may be concluded that they both have the mutation on the same gene and their intercourse would produce an albino offspring.
2. Mutant 3 x Mutant 7
See the table
Mutant 3
Mutant 7
1
+
+
2
+
+
4
+
?
5
+
?
6
-
?
8
?
?
9
+
+
10
+
+
The results of the mating between the mutant 3 and 7 are hard to predict since a lot of information is missing about other crosses.
Likewise, the results of other matings can be predicted.
Mutant 1
Mutant 5
2
-
-
3
+
+
4
+
+
6
+
+
7
+
?
8
+
+
9
+
+
10
+
+