Ch. 12 colonies indicate the insertion of foreign DNA within the polylinker site
ID: 256748 • Letter: C
Question
Ch. 12 colonies indicate the insertion of foreign DNA within the polylinker site of the plasmid 14. White vector, disrupting the lacz gene. A. True B. False 15. What is the advantage to producing a genomic library using phages instead of plasmids? A. Phage particle holds a much larger insert of foreign DNA B. Phage particle holds more inserts of foreign DNA C. Phage particle needs no bacterial host D. Phage particle produces a plaque E. All of the above 16. What's the main reason to covert mRNA to cDNA molecules in molecular cloning? A. DNA is more stable B. DNA is more catalytic C. mRNA is difficult to extract D. mRNA is easily contaminated E. All of the above 17. Which of the following processes is commonly used to introduce recombinant DNA molecules into eukaryotic hosts? A. Binary fission B. Conjugation C. Transduction D. Transfection E. Transformation 18. Which of the following processes uses a brief electric pulse to induce the formation of transient pores in the phospholipid bilayers? A. Electroporation B. Gene gun C. Microinjection D. Shuttle vectors E. Viral vectors 19. Which of the following methods is often used in gene therapy? A. Electroporation B. Gene gun C. Microinjection D. Shuttle vectors E. Viral vectors 20. It is generally easier to introduce recombinant DNA into prokaryotic cells than into eukaryotic cells. A. TrueExplanation / Answer
14.A) true
15.B) phage particle will holds more inserts of foreign DNA, as their genome is small in size.
16.A) DNA is more stable
17.D) trnasfection.
18.A) electroporation.
19.E) viral vectors
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