Problem 18.13 DNA RNA protein translation The two-hybrid assay works by coupling

Question

Problem 18.13 DNA RNA protein translation The two-hybrid assay works by coupling of enzyme assay or by colony growth. The two-hybrid assay involves the engineering of two hybrid genes. Each hybrid gene is a of the yeast GAL domain (GAL4-BD), and the other is fused to the GAL4 trans-activating domain (GAL4-AD) Expression of the reporter gene is controlled by the reporter gene expression depends on the binding of the binding to the activation of a reporter gene, whose expression can be detected by a simple colorimetric fusion of one of the proteins being studied to one-half activator. One protein is fused to the GAL4 DNA transcription replication translational regulatory sequence; therefore fusion protein to and activation requires that the protein fused to the domain transcriptional expressional structural functional interact with the protein fused to the only if the two hybrid proteins being studied. Proteins that that bind to each other weakly activate that bind tightly to each other activate Activation and reporter gene expression occur which depends on the binding of the two proteins do not activate reporter gene transcription, proteins reporter gene transcription, and proteins reporter gene transcription binding GAL4-AD UAS/GAL4 GAL4-BD do not bind to each other bind together low-level high-level

Explanation / Answer

1. Protein

2. DNA

3. Transcription

4. Translational

5. Transcriptional

6. Binding

7. UAS/GAL4

8. GAL4-BD

9. UAS/GAL4

10. Binding

11. Activation

12. Bind together

13. Do not bind to each other

14. Low-level

15. High-level

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---