Matching - For the following questions, match the genetic testing technique in t
ID: 270687 • Letter: M
Question
Matching - For the following questions, match the genetic testing technique in the left column with the timing of the test in the right column. Choices in the right column may be used once, more than once, or not at all. Genetic Testing Technique 13. Newborn screening E 14. Heterozygote screening Timing of Test a) After fertilization, before transfer of embryo to mother b) During embryonic or fetal development in the mother 15. Amniocentesis 16. Chronic villus sampling 17. Fetal cell sorting 18. Preimplantation genetic testing c) After birth Multiple Choice 19.) After the cycle of PCR, some fragments of the desired length have been obtained a) 1st b) 2nd d) 4th (e) 5t 20. The lysis solution contained which of the following? d) TE b) nucleotides c) DNA polymerase a) primers 21. The PCR step in each cycle that could occur at -61 degrees Celsius is typically... e) none a) denaturing b) elongation c) amplification d) annealing 22. The Alu repeat lab, DNA was found primarily where after the 1 centrifugation? d) neither of these c) both of these b) the pellet a) the supernatant True a, FalsebExplanation / Answer
Ans.13. Newborn screening is performed after birth of baby.
Ans.14. Hetrozygotic screening is performed after fertilization, before transer of embryo to mother.
Ans. 15. Amniocentesis is performed at the time of embryo development in the mother.
Ans. 16. Chronic villi sampling is performed at the time of embryo development in the mother.
Ans. 17. Fetal cell sorting is .performed at the time of embryo development in the mother.
Ans. 18. Preimplantation genetic testing is performed after fertilization, before transer of embryo to mother.
Ans. 19. In PCR cycles, desired fragments are obtained after the third cycle. Hence, the correct answer is option (c).
Ans.20. Some lysis buffer conatin TE (tris-HCL and EDTA) to manitain osmolarity and acidity of lysate. Hence, the correct answer is option (d).
Ans. 21. PCR includes denaturation (at 90°-95°C), annealing (at 56°-64°C), and extension (at 70°-74°C). Hence, the correct answer is option (d).
Ans.22. The Alu repeats are example of satellite DNA, which is found in supernatant after the first centrifugation. Hence, the correct answer is option (a).