Please explain + show work how this answer was determined. (handwritten response

Question

Please explain + show work how this answer was determined. (handwritten response preferred).

12. (12 points) Your Hfr strain is prototrophic and able to utilize the sugar lactose. However, it is sensitive to both streptomycin and kanamycin. Your F-strain has genotype lac-lys-kan str* tyr-. In the Hfr strain, the F+ plasmid integrated between the tyr and str genes, and tyr is the only marker transferred after 5 minutes of conjugation. a) What media should you use to select for the most exconjugants? Circle one answer for each sentence (3 points) Carbon source(s) on the master plate should be: glucose lactose The master plate should include: kanamycin streptomycin both The master plate should include: lysine tyrosine b both sugars no antibiotic

Explanation / Answer

Carbon source should contain is glucose.

Both antibiotics should be used in master plate.

Neither lysine nor tyrosine is used in master plate.

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---