Please answer questions 4 and 5 using the provided genotypes and answers from qu
ID: 271836 • Letter: P
Question
Please answer questions 4 and 5 using the provided genotypes and answers from questions 1-3... THANK YOU!!
Genotypes:
A1/A1: 80
A1/A2: 20
A2/A2: 90
Total: 190
1. Allele frequency estimation
Genotype
Freequency
Allele A1
Allele A2
Total
A1 A1
80
160
0
160
A1 A2
20
20
20
40
A2 A2
90
0
180
180
Total
190
180
200
380
Allele A1
= 180/380
0.47
Allele A2
= 200/380
0.53
2. Expected frequency estimation:
A1 A1
= 0.47*0.47 = 0.224
A1 A2
= 2*0.47*0.53= 0.499
A2 A2
= 0.53*0.53 = 0.277
3. Expected number estimation:
A1 A1
= 0.47*0.47 = 0.224 * 190
= 43
A1 A2
= 2*0.47*0.53= 0.499* 190
= 95
A2 A2
= 0.53*0.53 = 0.277*190
= 53
4. Use a X2 test to determine if your population sample conforms to the expected Hardy-Weinberg proportions. Provide a clear statement of how you would interpret the results of this test
5. If the population is in Hardy-Weinberg proportions, explain what that means. If the population does not appear to be in Hardy-Weinberg proportions, propose a mechanism that might account for the observed pattern and briefly explain your answer.
Genotype
Freequency
Allele A1
Allele A2
Total
A1 A1
80
160
0
160
A1 A2
20
20
20
40
A2 A2
90
0
180
180
Total
190
180
200
380
Explanation / Answer
4. Chi-square test:
Null hypothesis: The observed values are not deviating from the expected values.
Category
A1 A1
A1 A2
A2 A2
Observed values
80
20
90
Exprected Values
43
95
53
Deviation
37
-75
37
D^2
1396.399
5585.596
1396.399
D^2/E
32.76
58.96
26.53
118.25
X^2
118.25
Degrees of freedom
1
Inference: The calculated chisquare value i.e. 118.25 is greater than the table value i.e. 3.84 at 1 DF and 0.05 probability, hence the null hypothesis is rejected.
5. If the population is in Hardy-Weinberg proportions which means the population is in steady state and not evolving. If the population does not appear to be in Hardy-Weinberg proportions which means the population is not in steady state and the population is evolving .
Category
A1 A1
A1 A2
A2 A2
Observed values
80
20
90
Exprected Values
43
95
53
Deviation
37
-75
37
D^2
1396.399
5585.596
1396.399
D^2/E
32.76
58.96
26.53
118.25
X^2
118.25
Degrees of freedom
1