Assignment 1-Summer 2018 Share A AaßbCcDdEe AaBbCcDdEe Title Subtitle Normal No
ID: 272149 • Letter: A
Question
Assignment 1-Summer 2018 Share A AaßbCcDdEe AaBbCcDdEe Title Subtitle Normal No Spacing Heading 1 Heading 2 Pane d) Irreversible inhibitor (0.1 points) e) Reversible inhibitor (0.1 points) Answer 1) Inhibitor binds at the active site (0.1 points) te 3. Peniilin fits into the active site transpeptidases, enzymes that cross-ink components of the bacterial cell wall, and covalently binds to the site. A result of penicillin covalently binding to the active site of transpeptidases is that bacterial cell walls become fragile and the bacteria die a) Based on where penicillin binds to transpeptidase, what kind of inhibitor does it resemble? (0.3 JE points) Answer me ve he ed e. us on um b) How is the effect of such an inhibitor usually able to be rovernsed? (0.3 points) Answer c) Why would this approach not work with penicillin? (0.3 points) AnswerExplanation / Answer
d) Irreversible inhibitor: It is a inhibitor which chemically changes the enzyme structure in such a manner that the enzyme is not able to convert substrate into product.
e) Reversible inhibitor: It is a type of inhibitor which stops the enzyme from converting substrate into product; but once the inhibitor is removed, it allows the enzyme it was inhibiting to begin working again.
f) Inhibitor binds at the active site: It is an example of a competitive inhibitor.
3.a) Since penicillin binds to the active site, it is a type of competitive inhibitor.
3.b) The affinity for substrate to the active site of the enzyme can be increased by increasing the concentration of the substrate itself.
3.c) The binding of penicillin is irreversible. It inhibits the enzyme transpeptidase by reacting with a serine residue in the enzyme. This leads to termination of bacterial cell wall, and hence leads to death of the bacteria.