Please look closely. Need help with the following yellow boxes. ds? nois Probing

Question

Please look closely. Need help with the following yellow boxes.

ds? nois Probing Blot with anti-GFP primary antibody Calculate how much primary antibody you need to add to 5.0 ml of TBST buffer to give a 1:3333 dilution of the primary antibödy (mouse anti-GFP.) CALCULATION! HEIP 8. You will incubate your blot with primary antibody with another group (meaning 2 blots in one tube/tray at the same time with twice the volume of TBST+primary antibody). So, how much primary antibody you need to add to 10ml of TRST buffer to give a 1:3333 dilution of the primary antibody? 2TA00 a4HEIP anoe aa TeUM LA One of the two groups should use the Sml pipet at the bench to transfer 10.0 ml of TBST buffer into one of the "Falcon" conical tubes. (IMPORTANT: You will use the 10 ml pipet 9. for your cells in protocol #2) i o itsoq rninasi berior uoe foid oraroïq sh ni W "I 10. Bring this to your TA, who will pipette the calculated amount of antibody into the 10ml for

Explanation / Answer

1) you want a dilution 1:3333. it indicates that 3333 ul of buffer contains 1 ul of antibody.

3333 ul contains 1 ul of antibody

so 5000 ul contains (1/3333) *5000 = 1.5 ul antibody solution from stock.

and for 10 ml contains 3 ul of antibody solution from stock.

2)

you want a dilution 1:667. it indicates that 667 ul of buffer contains 1 ul of antibody.

667 ul contains 1 ul of antibody

so 5000 ul contains (1/667) *5000 = 7.5 ul antibody solution from stock.

and for 10 ml contains 15 ul of antibody solution from stock.

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---