The following results were obtained from a replica-plating experiment Complete m
ID: 273790 • Letter: T
Question
The following results were obtained from a replica-plating experiment Complete medium Glucose minimal salts (GMSA) GMSA+ streptomycin GMSA+ eucine GMSA + leucine + streptomycin GMSA +valine 36) Which colonies represent prototrophs? A) 4, 6, and 8 B) 5 and 6 C) 3 and 9 D) 1, 2,3, and 9 E) 4 and 8 37) Which colonies contain streptomycin resistant auxotrophs defective in the pathway for leucine synthesis? E) 3 and 9 A) 4 and 8 B) 5 and 6 C) 4, 6, and 8 D) 1, 2,3, and 9 38) Which colonies are streptomycin-resistant and valine-requiring? A) 3 and 9 B)I, 2, 3, and 9 C) 3, 5, and 9 D) 5 E) 3, 4, 8, and 9Explanation / Answer
Answere :
36. The option (C) is correct i.e. the colonies 3 and 9 are prototrophs which synthesize all essential nutrient without any need of external nutrients.
37. The option (A) is correct i.e. Colonies 4 and 8 are streptomycin resistant auxotroph which defective in leucine synthesis pathway.
38. The option (D) is correct i.e. colony 5 is streptomycin resistant and valine requiring.