In the course of sequencing a genome, a computer is trying to assemble the follo
ID: 273824 • Letter: I
Question
In the course of sequencing a genome, a computer is trying to assemble the following six DNA sequences into contigs (that is, stretches of contiguous sequences that can be obtained from overlapping clones): 5' AGCAAATTACAGCAATATGAAGAGATC 3' 5' AAAATGCCCTAAAGGAAATGAGATTTT 3 5 TGATCTCTTCATATTGCTGTAATTTGC 3 5 TCCTTTTAAAAATCTCATTTCCTTTAG 3 5' TACAGCAATATGAAGAGATCATACAGT 3' 5' AAATGCCCTAAAGGAAATGAGATTTTT 3 a. How many contigs are represented by this set of DNA sequences, and what is the sequence of each contig? b. Some of these sequences are complementary to each other in the region of overlap, while other sequences overlap but represent the same DNA strand. How is this possible? c. If these sequences are all derived at random from the human genome, why would you actually expect them not to overlap with each other?Explanation / Answer
contig sequences are those who are having overlapping sequences between them, That means some sequences are same as that of the other. Those are called contigs.Here for example i have
a. The second and last line represent one contig.
AAAATGCCCTAAAGGAAATGAGATTTT-AAATGCCCTAAAGGAAATGAGATTTTT
The one mark as bold are same so these are contigs.
first and last second
AGCAAATTACAGCAATATGAAGAGATC-TACAGCAATATGAAGAGATCATACAGT
here these are again contigs