I have given you a culture of NIH-L1 cells (Mouse Fibroblasts) and tell you I wa

Question

I have given you a culture of NIH-L1 cells (Mouse Fibroblasts) and tell you I want you to clone the whiskers gene (Wsk-1), which helps in whisker growth of a mouse. Using the plasmid below and in at least six, but no more than 10 steps, explain what you have to do to make a bacterial clone of the Wsk-1 gene. Assume you have the sequence of the entire gene. Please number each step. (10 Points) CMV promoter Bgill (13) Nrul (209) Mul (229) Ndel (485) 17 promoter Nhe I (896) Sspl (5310 Scal (4 SnaBI (591)Hindill (912) Ampicillin Pvd (4876 Acc551 (918) Kpnl (922) resistance Bam Hi (930) AspE) (4508) EcoRI (953) EcoRV (965) NotI (980) pcDNA3.1plus 5428 bp Xhol (986) Xbal (992) Apal (1002) BGH Poly-A Origin of replication F1 intergenic region

Explanation / Answer

The following steps can be taken in order to Wsk-1 gene in the mouse:

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