In which of the following techniques might a competitive inhibitor be used for p
ID: 275594 • Letter: I
Question
In which of the following techniques might a competitive inhibitor be used for purifying an enzyme? A. Hydrophobic interaction chromatography B. Affinity chromatography C. Gel permeation chromatography D. lon exchange chromatography E. Edman degradation Which technique could be used to determine the tertiary structure of a 200 kDa protein? A. X-ray crystallography B. Edman degradation C. gel permeation chromatography D. Nuclear magnetic resonance spectroscopy E. none of the above Which of the following statements about the Edman degradation technique is true? A. The Edman degradation procedure provides the sequence of a protein, by removing and identifying one amino acid residue at a time, beginning at the carboxyl terminal end B. The first step in Edman degradation is hydrolysis of the protein with 6M HCl at 110°C for 24 hr C. DNA sequencing is much more difficult and less efficient than amino acid sequencing by Edman degradation. D. Cyanogen bromide is useful for determining amino acid sequences, because it increases the number of Edman degradation cycles possible for proteins that lack methionine residues. E. Edman degradation is usually carried out with an automated instrument called a sequenatorExplanation / Answer
Ans 1. Option B
The immobilised ligand in the affinity chromatography is a substrate or competitive inhibitor of the enzyme.
Answer 2. Option A
X-ray crystallography is meant for determining the tertiary structure of such proteins.
Answer 3. Option e
Sequenator is an automatic machine used to carry on Edman degradation.