Please give a good explanation leading to the answer. 8 A molecular marker probe

Question

Please give a good explanation leading to the answer.

8 A molecular marker probe was used to study RFLPs. DNA was digested to completion with HinDIII and analyzed by a Southern blot using the probe. The restriction map is shown along with the position of the GGG probe. (More than one HindIlI site can/might be polymorphic in any one individual). Below the map is the RFLP pattern (on southern blots) obtained from individuals AA, BB, CC and DD. These individuals are homozygous for the RFLP locus. Using the map, identify the restriction site/s that are absent in individual AA, BB, CC and DD? 2.5 15 3.7 11.3 GGG probe cn n.1 17.5 15 32.5 11.3 3.7 30

Explanation / Answer

For AA = H1 and H2 might be polymorphic due to which HindIII cuts at H3,H4 and h5=

For BB= H1 ,H2, H4,H5 might be polymorphic. Thus the enzyme cuts at H3 and fragment size 17.5 and 15 are obtained.

For CC= H1 might be polymorphic

Fo DD= H3 H4 H1 might be polymorphic as 2 fragments of size 15 are obtained.

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