I) Which two sterile techniques allow you to separate cells in a culture into in
ID: 279877 • Letter: I
Question
I) Which two sterile techniques allow you to separate cells in a culture into individual colonies? Explain why these techniques can accomplish this separation. (4 pts) 2) In the inoculation experiment, do you expect growth in the control tube? Explain your answer. In your answer, include what it would mean if there were growth in the control tube. (2 pts) 3) Consider the following results: Strain Minimal medium no growth growth no growth Minimal medium + adenine no growth growth growth (a) Can strain 2 make its own adenine? Explain your answer. (2 pts) (b) Can strain 3 make its own adenine? Explain your answer (2 pts) (c) What situations might explain the results obtained with strain 1? Provide two different hypotheses. Consider both possible technical problems and possible nutritional requirements of the strain in formulating your hypotheses. Propose a nutritional test/experiment that could differentiate between the two possibilities. (4 pts)Explanation / Answer
1) the two techniques are dilution plating and streak plate method.
in dilution plating, you have to make a different dilution of the stock and plated onto suitable media plate. after an appropriate dilution of culture, you will get a plate having 50-100 isolated colonies.
In streak plate method, firstly divide a plate into 4-5 sections then you have to take a loop having culture and streak onto the plate at first section followed by sterilization of loop. after that touch, the loop to the first section streaked culture and streak to the second section. follow the same procedure for next sections. after proper incubation, you will get well-isolated colonies in 4-5 section.
2) the control tube is a sterilized medium without any organism inoculated in it. if there is a growth in control tube then it indicates that there is a contamination in the culture medium.