Streak Plate Isolation for Obtaining Pure Cultures Data Interpretation Please ty
ID: 280086 • Letter: S
Question
Streak Plate Isolation for Obtaining Pure Cultures Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. When an agar plate is inoculated, why is the loop sterilized after the initial inoculation is put on? 2. Define pure culture, a mixed culture? 3. Define a bacterial colony. List four characteristics by which bacterial colonies may be distinguished? 4. Why should a Petri dish not be left open for any extended period of time? 5. Why does the streaking method you used to inoculate your plates sult in isolated colonies? 6. Why is it necessary to isolate individual colonies from a mixed growth? 7. Why was blood agar, rather than a nutrient agar plate used for the culture from your mouth? 8. Are a large number of microorganisms found in the mouth a cause for concern? Explain. 9. How do microorganisms find their way into the mouth?Explanation / Answer
1) Ans-because to prevent unwanted contamination time of inoculation, so wire loop sterilized by flame heat
2)Ans- In mixed culture there is multiple type of bacteria present
pure culture- isolated from mixed culture. in this culture specialzed type of bacteria are grown.
3) bacterial colony is visible clump of bacteria grown on semi-solid media
characters- size, shape, colour, texure, elevation, margin
4) petri dish shoud not be open becaude to prevent unwaned contamination
5)streak plate metod gives specific specialized type of bacterial culture.
different type of colony distiguished based on practically
colony characteristics like size, shape, colour, elevation, margine, texure, gram nature