Describe how to make a 50 ml, 0.8% agarose gel with water, 40X TBE buffer, and a
ID: 280758 • Letter: D
Question
Describe how to make a 50 ml, 0.8% agarose gel with water, 40X TBE buffer, and agarose powder. Be sure to label/use units and show your work. (1 point) 6, 7. You have a highly concentrated stock solution, Sample R, which needs to be diluted into a working form. Illustrate a 10-fold serial dilution scheme that you would perform on Sample R to achieve a final working concentration that is 0.0001 times the concentration of the original stock solution. In your drawing, indicate the concentration of each tube as well as the amount of solute and solvent needed to create each dilution. If the solute is coming from a previous dilution, indicat? this with arrows. How many dilutions must you perform to obtain the final working concentration? Show your work. (2 points)Explanation / Answer
6.Usually 1x TBE is used for agarose gel electrophorosis (to prepare 1x from 40x TBE we have to take 2.5ml 40x TBE and mix it into 97.5 ml of water. 100*1/40=2.5)
To make 0.8% agarose gel in 50 ml take 0.4g agarose powder and dissolve it in 50 ml of 1x TBE buffer.
7.To achieve 0.0001 times dilution take containers and label them as 10-1 to 10-10 and then take 1ml solute from stock R and add in 9 ml solvent in 10-1 labeled tube and repeat this process till last tube. In this final set of tube 10-4 labeled tube contain our 0.0001 concentration.