CLINICAL APPLICATION activeness of an antibiotic. In this test, serial dilutions
ID: 281972 • Letter: C
Question
CLINICAL APPLICATION activeness of an antibiotic. In this test, serial dilutions of The broth dilution test can be used antibiotic were set up in the wells of a microtiter plate. Equal were added to each well. After incubation, the wells were examined for bacterial amounts of broth culture of Staphylococecus cureus of the growth. Wells with no growth were were recorded as (+) for growth and (-)for no growth subeultured in nutrient broth without the antibiotic. Results were reco Dilution Growth 1:10 through 1:70 1:80 1:90 1:100 1:200 through 1:500 1:10 through 1:150 1:160 1:170 1:180 1:190 through 1:500 What is the minimum bactericidal concentration of each antibiotic? What is the minimum bacteriostatic concentration? Which antibiotie is more effective against Staphylococcus aureus? Which antibiotic is more effective against Salmonella enterica? 192Explanation / Answer
What is the minimum bactericidal concentration of each antibiotic?
The data given in the experimental observation, it gives the following results:
The minimum bactericidal concentration of antibiotic A is 1:10 through 1:80, and for antibiotic B is 1:10 through 1:150.
So, the given blank can be filled with 1:10 through 1:80 for antibiotic A, and 1:10 through 1:150 for antibiotic B.
What is the minimum bacteriostatic concentration?
The data given in the experimental observation, it gives the following results:
The minimum bacteriostatic concentration of antibiotic A is 1:10 through 1:100 and for
antibiotic B is 1:10 through 1:160.
So, the given blank can be filled with1:10 through 1:100 for antibiotic A and
1:10 through 1:160 for antibiotic B.
Which antibiotic is more effective against Staphylococcus aureus?
The antibiotic A is more effective than antibiotic B against Staphylococcus aureus because it kills the bacteria better, and the growth was not seen even in sub culturing. This shows that antibiotic A kills much bacteria than B, and hence is more effective.
Which antibiotic is more effective against Salmonella enterica?
Data given in the table is not sufficient to clearly tell about the which antibiotic (A or B) is better against Salmonella enterica because as compared with the Staphylococcus aureus, it is gram positive bacteria and Salmonella enterica is gram negative bacteria.
The bactericidal mechanism against gram positive and gram negative bacteria is different. Hence it is not possible to compare effectiveness against Salmonella enteric.