I only need help with F, given the information provided for this problem and wha
ID: 282827 • Letter: I
Question
I only need help with F, given the information provided for this problem and what my answers are for the others parts! I know efficiency = Kcat/Km but I’m just not sure what numbers to use or if to include Ki somehow. For the enzyme with no inhibitor I got Km = 6.27 uM. And I’m very confused on the “increase or decrease of efficiency with a natural substrate”
Thank you for your help in advance!
4. Neuraminidase (EC 3,2.1.18) is the target of several anti viral therapeutics. An essential step in viral budding involves Neuraminidase hydrolyzing the glycosidic linkages of terminal sialic acid residues on cell-surface glycoproteins. Drugs that inhibit this hydrolysis prevent viral reproduction and thus the spreading of the virus through the human body. Neuraminidase is active as a homo-tetramer with four substrate-binding sites. Oseltamivir is a commercially available competitive inhibitor of viral Neuraminidase. It is marketed as Tamiflu and has a A Recently, researchers have attempted to design dual inhibitors that target both viral and bacterial Neuraminidase. The rationale is to prevent common secondary bacterial infections that accompany viral infection. Compounds A and B can inhibit both viral and bacterial Neuraminidase protein. The following data are representative of studies using Compounds A and B to inhibit viral Neuraminidase activity towards an artificial substrate (20-(4-methylumbelliferyl)-a-D-N acetylneuraminic acid]. Cleavage of this substrate releases a fluorescent product [methylumbelliferonel that can be quantified to determine enzyme activity. Each reaction was 1.0 ml and contained 2.5 pmol of recombinant viral neuraminidase ve (uM.s Substrate (? M) with 1 pM Compound A with 1 pM Compound B No inhibitor 15 20 25 .55 78 89 .91 1.01 41 63 72 0.85 93 .39 53 62 .68 0.72 a. Use these data to make a diagnostic Lineweaver-Burk plot. Include any tables, trendlines, equations, and a picture of the plot in your answer b. Using the plot in part a, determine the apparent Vmur and apparent Km for Compound A. What type of inhibitor is it? This inhibitr is campttitirt. c. Using the plot in part a, determine the apparent Vous and apparent K. for Compound B. type of inhibitor is it? . This is ndricm/ in inhibitrExplanation / Answer
Ans: It seems to be a general guessing type question by looking at the structure of / bonding pattern of natural substrates and artificial substrate. As some of the parameters are not given for the enzyme with a natural substrates like 6SLN-?2,6-sialyl-N-acetyllactosamine or 3SLN - ?2,3-sialyl-N-acetyllactosamine. Neuraminidase reported to be having high efficiency to natural substrates (because of ?2,3-linked sialic acid) than artificial substrate (MUNANA). Usally artificial substrates used for convenience and economics, here relative activity of an enzyme will be calculated than the activity with original natural substrate.
You may refer https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4358285/