I. To analyze genomic DNA restriction digests on a 0.8% agarose gel. When you ch
ID: 303069 • Letter: I
Question
I. To analyze genomic DNA restriction digests on a 0.8% agarose gel. When you checked the container with the 1X TAE electrophoresis buffer, you found it was empty. So you made 10 liters of 1X TAE Buffer, using the lab stock of 50 X TAE buffer solution, you found that the container with 50X TAE buffer was also empty. So now, you need to make 1000 mL of 5OX TAE Buffer. a. 1X TAE Buffer is 2mM EDTA in 40mM Tris with the pH adjusted to 8.0 with acetic acid. The formula weight for Tris is 121 g/mole. The formula weight for EDTA is 372 g/mole. To make 1,000mL of 50X TAE, you dissolved gTris and g EDTA in water. After adjusting the pH to 8.0 with acetic acid, water was added to a final volume of mL b. You made 10 Liters of 1X TAE buffer by pouringmL 50x liters of TAE into an empty container and then addingli water to bring the solution to a final volume of 10 LitersExplanation / Answer
To prepare 50X TAE buffer (Stock Solution)
The molecular weight of Tris acetate = 121
2.0 M Tris acetate= 2*121= 242 gm
The molecular weight of EDTA is 372 G/mole
0.5 M EDTA solution requires = 0.5 *372= 186 G/mole