Please help!! The average mass of the 20 common amino acids is about 125 daltons
ID: 3165157 • Letter: P
Question
Please help!!
The average mass of the 20 common amino acids is about 125 daltons each. Suppose an E. coli protein called your favorite protein (YFP) is 75 kilodaltons.
a. What is the length in nucleotides of the portion of the E. coli mRNA (i.e. the coding region) that is translated to make YFP?
b.The E. coli mRNA that makes YFP contains a 5’-UTR and a 3’-UTR. What is the function of the 5’-UTR and the 3’-UTR?
c.How many GTP molecules are required to translate 50 copies of YFP?
d.How many ATP molecules are required to translate 100 copies of YFP?
Explanation / Answer
the molecular mass on average for an amino acid is 125 daltons. So the number of amino acids in a protein of weight 75000 Dalton is given by 75000/125 = 600 amino acids. Or you can apply the formula molecular weight = (number of amino acid residues)*(average weight) - (number of peptide bonds)*(weight of water molecule)
Length = You can assume 3.5 Å per amino acid, 600*3.5 = 210 A