After, ion-exchange chromatography, we got the first result (1st photo). -cation

Question

After, ion-exchange chromatography, we got the first result (1st photo). -cation exchange chromatography -Asp, His, Arg -Buffer solution (pH 4.0, 8.5, 11.0) -filter paper & ninhydrin (110^•C oven)
And then using TLC, (silica gel plate), we got the second result. -spot (1st result material no.6, 7, 79, 80 + standard amino acid Asp, His, Arg) -Butanol:acetic acid:water = 12:3:5 -dry + ninhydrin (2% acetone) + dry + 110^•C oven

How can I understand the second picture? Isn’t it weird? 21 22 24 25 ?nt 32 28 McOKEUK

Explanation / Answer

Answer: TLC Chromatography technique used sationary & mobile phase. after dipped plate in solution of 24hr or more or less then solution reached the plate and after ninhydrin spray we can find amino acid, in 2 picture have Aspartic Acid,histidine,Arginine amino acid.we can seen by naked eye.

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