Map Suppose that a virulent form of pneumonia has appeared in North America and

Question

Map Suppose that a virulent form of pneumonia has appeared in North America and is spreading rapidly a young adults. A previously unknown bacterium has been isolated from several patients and is the suspected cause of the illness. The table below lists research techniques to begin a genetic characterization of the bacterium Research method Description and use density gradient centrifugation DNA molecules are centrifuged on a CsCl gradient, separating the molecules by density and allowing characterization of DN composition DNA microarray analysis Thousands of known DNA fragments are affixed to a glass slide at known locations and probed with sample DNA or RNA to detect the presence of specific sequences or to compare gene expression in two different conditions fluorescence in situ hybridization (FISH) Fluorescent probes designed to match a specific sequence are used to detect and localize the presence of the target sequence on chromosomes polyacrylamide gel electrophoresis (PAGE) DNA molecules move with an electrical radient through a porous gel, which all ow:s ster movement of smaller molecules, DNA fragments between 10-3000 bp are separated by size. PAGE is typically preceded by enzymatic digestion to break down larger DNA molecules pulse-field gel electrophoresis (PFGE) DNA molecules move through a gel with an electrical field, in which the voltage is constantly switched in three directions. PFGE separates DNA ranging in size from kilobases to megabases Continue below sedimentation-velocity centrifugation UV absorption is used to observe movement of

Explanation / Answer

1st question : 1 : Density gradient centrifugation

2 : Polyacrylamide gel electrophoresis

3 : Pulse field gel electrophoresis

2nd question : The following question can be answered with this information :

1- is the genome single stranded or double stranded ( since in double stranded A=T and G=C )

2- Does the genome have repetitive sequences ?

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