Correct answer: d. both lipase activity and fatty acid release Why is it difficu
ID: 3512201 • Letter: C
Question
Correct answer: d. both lipase activity and fatty acid release Why is it difficult to detect whether lipase is active in tube 57 You did not answer this question. Correct answer: d. The pH is already very low, so a decrease in pH might be difficult to detect Experiment Data Time Temp. 60 60 60 Tube No. Reagent 1 Lipase Lipase Lipase Deionized Water Reagent 2 Vegetable Oi Vegetable Oil Deionized Water Vegetable Oil Vegetable Oil Vegetable Oi Reagent 3 Bile Salts Deionized Water Bile Salts Bile Salts Bile Salts Bile Salts Reagent 4 PH 7.0 Buffer PH 7.0 Buffer H 9.0 Buffer pH 7.0 Buffer pH 2.0 Buffer pH 9.0 Buffer 37 37 37 37 37 pH 6.21 7.00 9.00 7.00 2.00 9.00 60 LipaseExplanation / Answer
The ONLY difference between constituents of test tube 1 & 2 is that test tube 1 contains bile salts and test tube 2 contains deionized water.
Bile salts are basic in nature. Bile salts are composed of the salts of four different kinds of free bile acids (cholic,deoxycholic, chenodeoxycholic, and lithocholic acids); each of these acids may, in turn, combine with glycine or taurine to form more complex acids and salts. Primarily two salts are sodium glycocholate and sodium taurocholate. So due to these salts the pH is basic.