Renal Physiology lab 1. Describe how the Loop of Henle and Collecting duct of th
ID: 3517717 • Letter: R
Question
Renal Physiology lab
1. Describe how the Loop of Henle and Collecting duct of the kidney work together to concentrate urine.
2. What is the effect of increased ADH (Antidiuretic Hormone) release on the collecting tube AND urine volume/concentration?
3. In the salt group, what can you conclude about the level of plasma osmolarity at the peak of salt excretion? What can you conclude about aldosterone level in the blood? ADH level?
4. Describe how the osmoreceptors lead to increased urine volume in the water only group
Explanation / Answer
Q 1 loop of henle an collecting duct both work together by countercurrent multiplier mechanism-
In descending lim of loop of henle water moves out via aquaporin 1, so inside fluid become hypertonic and osmolarity at tip is 1200mosm/kg. While thick ascending LOH Na cl and k moves out via Na k 2cl transporter because water left inside fluid become hypotonic to plasma. The osmolarity of interstitium maintain at 1200 by urea diffuse from collecting duct. Urea diffuse in ascending limb of LOH and further cincentrate urine...cycle countinue. And urine concentrated.
2 ADH effect it increse water absorption by aquaporin channel formation in collecting duct in principal cell it decrease urine volume and concentrated the urine.
3 level of plasma osmolarity should be maintain at more lower level than urinary salt excretion because to excrete that same amount of high osmolar solute body required more amount of water to to excrete that salt.
Level of both ADH & Aldosterone are incresed to prevent excess water loss...for salt excretion.
Q4 osmoreceptor present in the brain hypothalamus they stimulated when blood osmolarity increases and they relese ADH to posterior pitutary and water is conserved ...in water only people level of ADH is low.