Can someone please answer the following questions for me in depth Results 1. Use
ID: 3729666 • Letter: C
Question
Can someone please answer the following questions for me in depth Results 1. Use the computer system to produce: the TIC chromatogram of each run experimental and standard mass spectra for computer matching of each peak with intemal library spectra. a) b) If time permits, produce selected mass spectra in tabular form and use eight-peak index for identification. 2. Formative Questions Which type of compounds can be analysed by GC-MS and indicate examples of compounds that would require derivatisation prior injection into GC-MS? 1. 2. Comment on the characteristics of the chromatographic column that could affect the separation of peaks. Which species can be detected in MS and how are they generated? 3. 4. Which type of mass analyser has been used? briefly explain how it operates 5. How good is GC-MS at distinguishing between isomers? Compare GC-MS with GC-FID and other GC detection systems. What advantages and disadvantages do each of them offer? 6. 19Explanation / Answer
GC Means Gas chromatography(GC) and MS Means mass spectrometry(MS)
1)
explosive compounds such as terpenes are preferably inspect by GC/MS.
high temperature in GC makes its use confined stable volatile compounds which form a small number of Natural products.
The GC indicator uses a detector to measure the different compounds like flame ionization detector, cross section detector and thermal conductivity detector, etc.We can choose proper detector depends upon the use.
We use GC-MS due to its flexibility and cost.
Gas Chromatography and Mass Spectrometry analysis can be performed on liquids, gases or solids
GC-MS is generally used in pharmaceutical industries for systematic research and development,quality assurance,production and quality control.GC-MS provides opportunity to perform the analysis of new compounds for characterization and identification of synthesized or derivatized compound.
2)
The GC instrument uses a detector to measure the different compounds as they emerge from the column.Some characteristics are that the flame detectors destroy the sample, the thermal conductivity detector is universally sensitive and the argon ionization detector requires argon as a carrier gas.
3)
Generally MS will display a peak for the unfragmented molecule of the specimen is greatest mass detected.it is used to fit the pieces together from the other peaks in the mass spectrum(MS) and disclose the mass of the molecule.
generating the molecular mass, the technician makes an educated suppose of the species identity and compares the mass spectrum to reference spectra for confirmation.Computers are commonly used for spectral analysis.
4)
mass spectrometry(MS) is an important method for identifying the atoms or molecules in complex chemical substances.it is used his machine to prove the existing of many normally happening isotopes.
MS are operates in four stages in a mass spectrometer those are ionisation, acceleration, deflection, and detection.