I need opinions for a project I am attempting to develop. Unfortunately, I don\'

Question

I need opinions for a project I am attempting to develop. Unfortunately, I don't remember as much chemistry as I hoped Idid. I need a series of three molecules that will interactwith amino acid residues, preferably covalent bonds.

I was hoping to do this with modified nucleotides.
http://www.trilinkbiotech.com/products/ntp/list_basemodribo.asp
http://www.berryassoc.com/display.asp?string=modified&Submit=GO&results=display.asp%3F&pagefrom=fresh
These are some I have found.

Can anyone tell me which ones would be best for interacting with anactive site that consists of 2x Glu, Asp, Lys, and His? Iwant to add them within an RNA molecule and the interaction withthe active site will prevent endonucleolytic cleavage. Any inputwill be helpful.

Explanation / Answer

      I'm not sure of theanswer, but I'm trying to answer it. Kindly ignore if notappropriate.       The 3 molecules that willbest interact with the active site that consists of 2x Glu, Asp,Lys, and His are:       O-Methyl-2-aminoadenosine-5'-triphosphate and O-Methyl-5-Methyluridine-5'-triphosphate:       These two moleculescan interact with either Glutamate or Aspartate.       These two amino acids areacidic amino acids with negative charge at their side chain.       So these forms a covalentbond with the given compound.       2-Aminoadenosine-5'-triphosphate can covalently attach to the Lysine orHistidine amino acids in the active site.       I think these cannot beattacked by the endonucleases as they do not have phosphodiesterbond.       Once again I remind youthat I'm not sure of the answer but tried to help you out.       Hope it will be of someadvantage.

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