Map pling order the steps of the Southern blot procedure as they would be applie

Question

Map pling order the steps of the Southern blot procedure as they would be applied to DNA fingerprinting analysis. First step For step 1, keep in mind that DNA fragments Denature DNA by soaking gel in NaOH. are fractionated by gel electrophoresis. Separate DNA fragments by gel electrophonesis. Cleave DNA using restriction endonucleases. Incubate membrane with radioactively labeled ssDNA probe. Transfer ssDNA to a nylon or nitrocellulose membrane. Place film onto membrane to produce autoradiogram. Last step

Explanation / Answer

1. Cleave DNA using restriction endonucleases.

2. Separate DNA fragments by gel electrophoresis.

3. Denature DNA by soaking gel in NaOH.

4. Transfer ssDNA to a nylon or nitrocellulose membrane.

5. Incubate membrane with radioactively labeled as ssDNA probe.

6. Place film onto membrane to produce autoradiogram.

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