Include the report sheet. Summary of your results. Also, answer the discussion q
ID: 491502 • Letter: I
Question
Include the report sheet. Summary of your results. Also, answer the discussion questions below: Which is better suited to separate and detect small amounts of material: (a) column chromatography or (b) paper chromatography? Explain. Compound X has an Rf value of 0.25. How far will compound X have traveled from the origin when the eluent front has traveled 4 cm? How can you tell if different manufacturers used the same or different ink to produce the same color pens? Based on the R_f values you obtained for the phospholipids, which of them migrates faster and which property influenced the rate of migration? Did it support your initial prediction? Consider the following compounds. Assuming these compounds can be separated by TLC, list them by increasing value of R_fExplanation / Answer
1. paper chromatography is better to detect small amount of compound
2.Distance traveled by a spot/distance traveled by solvent = 0.25
or, distance travelled by the spot = 0.25 * 4cm = 1 cm
3. ink is mixture of organic compound. A TLC exteriment will given th information
4. Greater the polarity of the compound stronger will it bind with the TLC plate and as a result will move slower. Non-polar compound will move faster and will have highest Rf value.
5. b has a greater polarity. Rf (a) > Rf(b)