Construct a Calibration graph of Concentration VS Absorbance with a trendline eq
ID: 537232 • Letter: C
Question
Construct a Calibration graph of Concentration VS Absorbance with a trendline equation. Absorbance of Diluted Commercial Tablet Calc. Cone. (M) of Diluted Commercial Tablet (100 mL) Calc. Cone. (M) of initial Commercial Tablet (250 mL) Moles of Aspirin in Tablet (mol) Mass of Aspirin in Tablet in milligrams (mg) Commercial Aspirin tablets used in this lab contained 325 mg of active ingredient. Calculate the percent difference of your result vs this true value. % difference = (Measured-True)/True * 100% If DI water was used to calibrate the spectrometer instead of iron (III) chloride solution how would this have affected the reported absorbance of the solutions? (Increased, Decreased, unaffected) Briefly explain. If finger prints were not removed for the sample cuvettes before measuring how would this have affected the reported absorbance of the solutions? (Increased, Decreased, Unaffected) Briefly explain.Explanation / Answer
Aspirin analysis
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2) If only DI water is used for calibration the resultant absorbance would be addition of absorbance due to complex formed and the absorbance due to Fe(III) species. Thus the reported absorbance would be higher (increased) than the expected value.
3) Finger prints does not allow the light to pass throught the cell. Opaque cells lead to lower absorbance values as less amount of light is being passed into the solution.