Site directed mutagenesis allows one to introduce virtually any desired mutation
ID: 53873 • Letter: S
Question
Site directed mutagenesis allows one to introduce virtually any desired mutation in a specific gene. One very useful application of the technique involves modifying a particular protein and evaluating the effect on biological or chemical activity. In the past, two methods have been used for producing modified proteins. One is to use chemical agents or ultraviolet light to induce mutations that result in changes in the amino acid sequence of proteins. The other is to modify certain residues in an isolated protein by treatment with chemical reagents; an example is the inactivation of a reactive serine in the active site of proteolytic enzymes like chymotrypsin, using diisopropylflurophosphate.
Explanation / Answer
Yes site directed mutagenesis allows to introduce mutation in a specific gene using cloning.
Several proteins are modified for enhanced properties such as stability in various non physiological environments such as extreme pH, temperature, salinity e.t.c.