QUESTION 34 You wish to amplify the following double stranded DNA segment using
ID: 53945 • Letter: Q
Question
QUESTION 34
You wish to amplify the following double stranded DNA segment using PCR. The following sequence represents one of the two strands of the duplex molecule, which has an antiparallel complement (not shown). What are the sequences of the 2 oligonucleotide primers (5' to 3') that you would use to amplify this entire DNA segment? Note that the Tm values of the primers should be similar.
5'- ACGTGCCTCTCCTCATACGAGGATATATATAGTGTTATTTTGTTTGGGGTGTTTGTTTTGGATTGT
GTTTTTTTGTTTATTTCGTGTGTTATGTAACATATGACGTGCCTCTCCTCATATAATTTCTATCTACTT
GCACAATGAAATATATTCATAAAGCATAAGCTCATTG-3'
QUESTION 35
The infection cycle of a retrovirus includes all EXCEPT:
Assembly and release of virus particles.
Binding of virus to the host cell surface.
Integration of the viral DNA into the nuclear chromosomal DNA as a provirus.
Reverse transcription of viral RNA genome to make double stranded DNA.
Transcription of viral mRNA using the viral RNA genome as template.
3 points
QUESTION 36
Restriction endonuclease enzymes:
Are used along with a DNA methylase enzyme to cleave DNA in vitro.
Always generate single-stranded overhangs, or “sticky ends”.
Hydrolyze a phospho-ester bond on both strands of a double stranded DNA molecule.
Add methyl groups to adenines or cytosines that occur in a particular context.
Digest DNA progressively from the 3’ to 5’ end to yield nucleotide monophosphates.
3 points
QUESTION 37
Genetic complementation by crossing haploid mutant yeast cells can be used to:
Determine whether the mutations in the haploid parent cells are dominant.
Determine whether the recessive mutations in the haploid parent cells occur in the same gene.
Create dominant negative mutants.
All of the above.
38. Indicate for each statement if is true for eukaryotic cells, prokaryotic cells, or both.
A.
both
B.
prokaryotic cells
C.
eukaryotic cells.
Genes are often polycistronic (more than one translation start site on a single mRNA).
Genes often contain introns that are removed post-transcriptionally by mRNA splicing.
Genes can be found on both strands of the chromosomal DNA.
complex transcription units can generate multiple mRNAs via alternative polyadenylation.
Translation typically can start at multiple ribosome binding sites on mRNAs.
Genomes can contain transposons.
Assembly and release of virus particles.
Binding of virus to the host cell surface.
Integration of the viral DNA into the nuclear chromosomal DNA as a provirus.
Reverse transcription of viral RNA genome to make double stranded DNA.
Transcription of viral mRNA using the viral RNA genome as template.
Explanation / Answer
QUESTION 34:
QUESTION 35.The infection cycle of a retrovirus includes all EXCEPT:Integration of the viral DNA into the nuclear chromosomal DNA as a provirus.
QUESTION 36.Restriction endonuclease enzymes:Hydrolyze a phospho-ester bond on both strands of a double stranded DNA molecule.
QUESTION 37.Genetic complementation by crossing haploid mutant yeast cells can be used to:Determine whether the recessive mutations in the haploid parent cells occur in the same gene.
QUESTION 38.
Genes are often polycistronic (more than one translation start site on a single mRNA): prokaryotic cells
Genes often contain introns that are removed post-transcriptionally by mRNA splicing:eukaryotic cells
Genes can be found on both strands of the chromosomal DNA:prokaryotic cells and eukaryotic cells
complex transcription units can generate multiple mRNAs via alternative polyadenylation:eukaryotic cells
Translation typically can start at multiple ribosome binding sites on mRNAs:prokaryotic cells
Genomes can contain transposons:prokaryotic cells and eukaryotic cells