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Please help me solve this problem. # of colonies solution plated amount on plate

ID: 5425 • Letter: P

Question

Please help me solve this problem.
# of colonies solution plated amount on plate (mL) cells per mL 700 whole milk 0.1 85 0.1 mL milk in
9.9 mL water 1.0 10 0.1 mL milk in
9.9 mL water 1.0
Please help me fill out the last column of this table (cellsper mL), and please explain how you calculated the answers.
# of colonies solution plated amount on plate (mL) cells per mL 700 whole milk 0.1 85 0.1 mL milk in
9.9 mL water 1.0 10 0.1 mL milk in
9.9 mL water 1.0
Please help me fill out the last column of this table (cellsper mL), and please explain how you calculated the answers. # of colonies solution plated amount on plate (mL) cells per mL 700 whole milk 0.1 85 0.1 mL milk in
9.9 mL water 1.0 10 0.1 mL milk in
9.9 mL water 1.0

Explanation / Answer

AS far as your data presents the problem is very simple, In the first case, 0.1ml gives you 700 colonies which means700 bacterial cells. Therefore 1ml will have 700/0.1 = 7000 cells. in the second case, you have plated 1ml and you have got 85colonies. This indicates that you have 85 cells per ml. In the third case, you have plated 1ml and you got 10colonies. This indicates that you have 10cells per ml. kindly PM me if you have any other doubts. I willclarify.