I need solutions for 7 and 8. thanks 7. You have a protein of an unknown concent
ID: 555621 • Letter: I
Question
I need solutions for 7 and 8. thanks
7. You have a protein of an unknown concentration but you know that the range is 0.2 mg/mL to 2 know, that typically amounts around 10 g give and 9 others). Using 5 wells, how would you design an experiment to look at the effect of amount of protein loaded on the band appearance on the gen Remember that you are mixing 10 of sample buffer w protein sample You will need to make 5 different dilutions of the original protein mg/mL You a good result on an SDS-PAGE gel. You have 9 wells (1 ladder th 30 uL of solution. How will you do that? 8. Find a picture of the ladder/marker we are using for the experiment. Draw a figure of a gel with 10 lanes with the ladder/marker (the one we are using) in the first lane. Label the lanes on the marker lane. In lane 2 draw what you expect a mixture of proteins to look like (let's say there are 1000 different proteins with molecular weights between 2 kD and 130 kD). Think about a smear rather than individual bands. In lane 3 draw what a monomeric 18 kD protein would look like. In lane 4, draw what it would look like if the protein in well 3 dimerized. In lane 5, draw what it would look like if there were both monomers and dimers of the 18 KD protein present Remember DTT and 2-mercaptoethanol reduce disulfide bonds and make monomers out of dimers etcExplanation / Answer
A 1 mg/mL BSA standard solution is provided. This protein will be used to create a standard curve that spans a range of 2-20 µg/mL. Obtain fourteen 1.5 mL Eppendorf tubes. Label two “0”, two “2”, two “4”, etc. to match the number of µL BSA added, as indicated in Table 1. The numbers in the BSA column also reflect the µg/mL in each sample. Every sample must be done in duplicate to ensure an accurate standard curve.