Plz answer both, that would be really helpful! QUESTION 5 1 points In order to o

Question

Plz answer both, that would be really helpful! QUESTION 5 1 points In order to obtain the irue ('corrected") luorescence spectra, the response (arbitrary units, a.u.) of a O normalized to the corresponding absorbances O calibrated for a given combination oflight sou O adjusted according to the pH value of cacih sample ofl s to be: tector, and monochromators QUESTION 6 1 points Save a Inner filter effect refers to O distortions in the fluorescence spectra (decrease in amplitude and change in shape) for concentrated (A-0.1) samples O distortions in the fluorescence spectra when an optical filter (rather than a monochromator) is used to produce the excitation light O dramatic decrease in noise (improvement in signal-to-noise ratio, S/N) in fluorescence spectra when a liquid nitrogen-cooled CCD is used as a detector

Explanation / Answer

I order to obtain the true ("corrected ") fluoresecnce spectra, the response of a spectrofluorometer needs to be Calibrated for a given comination of light source, detector and monochromators.

Inner filter effect refer to : Distortions in the fluoresence spectra when an optical fibre is used to produce excitation light.

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