An oligomeric protein has a molecular weight of 429000. A 760 mg sample was trea

Question

An oligomeric protein has a molecular weight of 429000. A 760 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene (Sanger's reagent) until the reaction was complete. Next the peptide bonds were hydrolyzed by heating with concentrated HCI. The product of the complete hydrolysis contained 3.5 of the compound shown below, and no other 2,4-dinitrophenyl derivatives of the -amino groups of other amino acids could be found. NO2 How many polypeptide chains are in this protein? (Enter your answer as a whole number) CH2 Number

Explanation / Answer

mmoles of 2,4-dinitrophenyl derivative = mass / molar mass

                                                          = 3.5 / 256

                                                           = 0.01367 mmol

mmoles of protein = 760 / 429000 = 1.77 x 10^-3

number of poly peptides bonds = 0.01367 / 1.77 x 10^-3

                                                  = 7.72

number of poly peptides bonds = 8

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