Assume the two E.coli strains shown below are allowed to conjugate. Hfr strain:

Question

Assume the two E.coli strains shown below are allowed to conjugate. Hfr strain: pro+, arg+, his+, lys+, met+, ampicillin-sensitive F– strain: pro-, arg+, his-, lys-, met-, ampicillin-resistant pro = proline; arg = arginine; his = histidine; lys = lysine; met = methionine What supplements would you add to glucose minimal salts agar to select for recombinant F– cells that are lys+ and met+?

lysine, methionine

proline, histidine, arginine

ampicillin, lysine, methionine

ampicillin, proline, histidine, arginine

ampicillin, proline, histidine

lysine, methionine

proline, histidine, arginine

ampicillin, lysine, methionine

ampicillin, proline, histidine, arginine

ampicillin, proline, histidine

Explanation / Answer

D. ampicillin, proline, histidine, arginine

Fecombinant F– cells, means ampicillin resistant (so, add ampicillin); it is lys+ and met+, so no need to add them, we have to add proline, histidine, arginine.

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