Part B presses the A protein subunit from an enzyme is part of a research study

Question

Part B presses the A protein subunit from an enzyme is part of a research study and needs to be characterized. A total of 0.160 g of this subunit was dissolved in enough water to produce 2.00 mL of solution. At 28 °C the osmotic pressure produced by the solution was 0.138 atm. What is the molar mass of the protein? rams f moles perimentally of the ponding roperty of sion and re frequently oles in on. Express your answer with the appropriate units. View Available Hint(s) l to Value Units erature, Kf is n constant, solute per Submit Previous Answers X Incorrect; Try Again; 4 attempts remaining

Explanation / Answer

Osmotic pressure = C.R.T

where C is the concentration

R = 0.0821 L.atm/mol.K

T = 28oC = 301.15 K

So putting all the values we get :

0.138 = C x 0.0821 x 301.15

C = 0.0056 M

C = number of moles of protein / volume of solution in L

0.0056 = n / 0.002

n = 0.0000112 mol

So the molar mass of the protein = 0.160 / 0.0000112

= 14285.7 g/mol

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